Search results for "genetics [Transcriptome]"

The Influence of the Proton Gradient on the Activation of Ferredoxin-NADP+-oxidoreductase by Light

1988

Ferredoxin-NADP+-oxidoreductase (FNR, EC 1.18.1.2) has been shown to be activated by light within a few seconds during dark-light transitions and inactivated in the dark. In previous papers this could be pointed out by the correlation of cytochrome f induction kinetics to the rate of NADP-photoreduction and the variable fluorescence. The present study deals with the role of the proton gradient during the activation process. The transition from an inactive to an active form is followed continuously in an in situ system. The steady-state rate of NADP-photoreduction is affected only by ionophores which inhibit a formation of the proton gradient, but not by inhibitors of the electric field. It …

Growth and Photosynthesis of Nanochlorum eucaryotum, a New and Extremely Small Eucaryotic Green Alga

1982

Nanochlorum eucaryotum is a very small species of unicellular coccoid green algae (1.5 pm). The growth of Nanochlorum under different conditions of salinity, pH and light intensities was studied. Optimal growth rates were observed with normal sea water salinity and low light conditions at pH 7.0. The contents of chlorophylls, carotinoids, soluble proteins and the chlorophyll a to chlorophyll b ratio were measured. The light saturating curves of Nanochlorum cells grown under light intensities of 100 lx, 2000 lx and 10000 lx reveal a very narrow capacity of light adaptation. When cultured under higher light intensities, Nanochlorum was not able to reach high photosynthetic activities but unde…

9-Methyl-3-phenyldiazenyl-9H-carbazole: X-ray and DFT-calculated structures

2006

The title compound, C19H15N3, was prepared by condensation of 3-nitroso­carbazole and aniline with subsequent methyl­ation. The structure is built up of stacks of almost planar mol­ecules. Density functional theory (DFT) calculations predict a completely planar conformation, different from that observed in the crystal lattice. HOMA (harmonic oscillator model of aromaticity) indices, calculated for three aromatic rings, demonstrate the small influence of the azo substituent on π electrons in the carbazole system.

Single Molecule Spectroscopy: Methodological Developments and Experiments at Low Temperature

2001

The involvement of DNA damage response pathway in nuclear reorganization during netotic initiation

2019

Overcome Chemoresistance: Biophysical and Structural Analysis of Synthetic FHIT-Derived Peptides.

2021

The fragile histidine triad (FHIT) protein is a member of the large and ubiquitous histidine triad (HIT) family of proteins. On the basis of genetic evidence, it has been postulated that the FHIT protein may function as tumor suppressor, implying a role for the FHIT protein in carcinogenesis. Recently, Gaudio et al. reported that FHIT binds and delocalizes annexin A4 (ANXA4) from plasma membrane to cytosol in paclitaxel-resistant lung cancer cells, thus restoring their chemosensitivity to the drug. They also identified the smallest protein sequence of the FHIT still interacting with ANXA4, ranging from position 7 to 13: QHLIKPS. This short sequence of FHIT protein was not only able to bind …

Volatile constituents of aerial parts of Lasiopogon muscoides

2009

IL-23-mediated mononuclear phagocyte crosstalk protects mice from Citrobacter rodentium-induced colon immunopathology.

2014

Gut homeostasis and mucosal immune defense rely on the differential contributions of dendritic cells (DC) and macrophages. Here we show that colonic CX3CR1+ mononuclear phagocytes are critical inducers of the innate response to Citrobacter rodentium infection. Specifically, the absence of IL-23 expression in macrophages or CD11b+ DC results in the impairment of IL-22 production and in acute lethality. Highlighting immunopathology as a death cause, infected animals are rescued by the neutralization of IL-12 or IFNγ. Moreover, mice are also protected when the CD103+ CD11b− DC compartment is rendered deficient for IL-12 production. We show that IL-12 production by colonic CD103+ CD11b− DC is r…

SAT0023 Artery Tertiary Lymphoid Organs Occur in Giant Cell Arteritis

2016

Background Arteries are immuno-privileged sites. In advanced atherosclerotic lesions, however, adventitial lymphoid infiltrates, sometimes aggregated in lymphoid follicles (the so called artery tertiary lymphoid organs, ATLO), occur together with marked neoangiogenesis and lymphangiogenesis, and with the extensive induction of high endothelial venules. Objectives To investigate if tertiary lymphoid organs (TLO) are present in GCA and their formation is associated with the ectopic expression of constitutive lymphoid tissue-homing chemokines. Methods RT-PCR, immunohistochemical and immunofluorescence analysis were used to determine the presence of ectopic TLO in GCA and the expression of chem…

Testing <em>Drosophila</em> Olfaction with a Y-maze Assay

2014

Detecting signals from the environment is essential for animals to ensure their survival. To this aim, they use environmental cues such as vision, mechanoreception, hearing, and chemoperception through taste, via direct contact or through olfaction, which represents the response to a volatile molecule acting at longer range. Volatile chemical molecules are very important signals for most animals in the detection of danger, a source of food, or to communicate between individuals. Drosophila melanogaster is one of the most common biological models for scientists to explore the cellular and molecular basis of olfaction. In order to highlight olfactory abilities of this small insect, we describ…